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Zhao, Z, et al., Metabolite Profiling of 5'-Amp Induced Hypometabolism. Metabolomics, 2013.

ABSTRACT


We have previously demonstrated that50-adenosine monophosphate (50-AMP) can be used toinduce deep hypometabolism in mice and other nonhibernatingmammals. This reversible 50-AMP inducedhypometabolism (AIHM) allows mice to maintain a bodytemperature about 1 C above the ambient temperature forseveral hours before spontaneous reversal to euthermia.Our biochemical and gene expression studies suggestedthat the molecular processes involved in AIHM behaviormost likely occur at the metabolic interconversion level,rather than the gene or protein expression level. Tounderstand the metabolic processes involved in AIHMbehavior, we conducted a non-targeted comparative metabolomicsinvestigation at multiple stages of AIHM in theplasma, liver and brain of animals that underwent AIHM.Dozens of metabolites representing many important metabolicpathways were detected and measured using ametabolite profiling platform combining both liquid-chromatography–mass spectrometry and gas-chromatography–mass spectrometry. Our findings indicate that there is awidespread suppression of energy generating metabolicpathways but lipid metabolism appears to be minimallyaltered. Regulation of carbohydrate metabolites appears tobe the major way the animal utilizes energy in AIHM andduring the following recovery process. The 50-AMPadministered has largely been catabolized by the time theanimals have entered AIHM. During AIHM, the urea cycleappears to be functional, helping to avoid ammonia toxicity.Of all tissues studied, brain’s metabolite flux is theleast affected by AIHM.

Keywords
Metabolomics Hypometabolism  50-AMP Non-targeted  Plasma Liver

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